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crystals of HUMAN TOPOISOMERASE II-DNA binary complex, methods for preparing the same and uses thereof |
刊登日期:2014/05/21 |
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‧ 專利名稱 |
crystals of HUMAN TOPOISOMERASE II-DNA binary complex, methods for preparing the same and uses thereof |
‧ 專利證書號 |
US8765443 B2
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‧ 專利權人 |
國立臺灣大學 |
‧ 專利國家
(申請日) |
美國 (2012/07/24)
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‧ 發明人 |
詹迺立, 李財坤, 吳權娟, 王英任, |
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技術摘要: |
本發明揭露一種人類第二型拓樸異構酶(hTOPII)-DNA二元複合體、其製備方法及其應用。hTOPII-DNA二元複合體包含含有hTOPII核心區域(hTOPII core )之hTOPII部位及與hTOPII部位複合之合成雙股DNA。合成雙股DNA具有第一股DNA,其包含核苷酸位置3至20之5'-NNNCCGAGCNNNNGCTCGGNNN-3'序列(SEQ ID.NO.:1),其中N為腺嘌呤、胸腺嘧啶、胞嘧啶或鳥嘌呤,以及與第一股DNA互補之第二股DNA。
Disclosed herein are a crystal of a human TOPII (hTOPII)-DNA binary complex, the method for preparing the same and the use thereof. The hTOPII-DNA binary complex includes an hTOPII portion that contains an hTOPII core domain (hTOPIIcore), and a synthetic double-stranded DNA in complex with the hTOPII portion. The synthetic double-stranded DNA has a first DNA strand comprising nucleotide positions 3 to 20 of the sequence of 5′-NNNCCGAGCNNNNGCTCGGNNN-3′ (SEQ ID NO: 1), wherein N is any one of adenine, thymine, cytosine, or guanine, and a second DNA strand complementary to the first DNA strand.
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聯繫方式 |
聯絡人:
研發處產學合作總中心 |
電話:
(02)3366-9949 |
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地 址:
10617臺北市大安區羅斯福路四段1號 禮賢樓六樓608室 |
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