可表現黑色素之真菌轉殖株及其方法
  ‧ 專利名稱 可表現黑色素之真菌轉殖株及其方法
  ‧ 專利證書號 I437096
US 8,518,393
  ‧ 專利權人 臺大、農委會(主導)
  ‧ 專利國家
    (申請日)
中華民國 (2009/12/31)
美國 (2010/11/26)
  ‧ 發明人 曾敏南, 曾顯雄,
 
技術摘要:
本發明由可產生黑色素(melanin)之磚格孢菌(Alternaria alternata)中選殖出黑色素生合成相關基因群,包括聚酮合成酶(Polyketide synthase,PKS)、小柱孢酮脫水酶(Scytalone dehydratase,SCD)及1,3,8-三羥基萘還原酶(1,3,8-trihydroxynaphthalene reductase,THN)等基因。將該基因建構於轉殖載體pCAMBIA 1300,再經由農桿菌(Agrobacterium tumefaciens)媒介轉殖至黑殭菌(Metarhizium anisopliae),使其表現該基因並產生黑色素,進而提高黑殭菌耐受紫外線照射的能力。並經由多種途徑證實黑色素生合成基因在轉殖株中被轉錄及表現,並且顯著的提高轉殖株(transformant)對紫外線、乾燥及高溫之耐受能力,以及在此UV輻射逆境下對寄主昆蟲有較高之侵染能力。

The present invention cloned melanin biosynthesis genes encoding polyketide synthase (PKS), scytalone dehydratase (SCD) and 1.3.8-trihydroxynaphthalene reductase (THN) from the dematiaceous Alternasia alternate into plasmid pCAMBIA1300, followed by transformation of the plasmid into Matarhizium anisopliae via Agrobacterium tumefaciens-mediated transformation. The transformant was able to express the abovementioned genes and synthesize melanin, which then showed enhanced UV tolerance. The transcription and expression of these melanin genes were confirmed in several pathways. The tolerances toward UV radiation, drought and high temperature were increased significantly in these transformants. In addition, the host insects were more susceptible to these transformants under UV radiation.



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