 技術摘要 / Our Technology： |
本發明係關於一種提昇生物反應器生產抗病有用蛋白產量之方法，其包含下列步驟：(a)構築一質體，其至少包含一外源抗病蛋白之核酸序列與一α肌動蛋白啟動子；(b)將構築之質體轉殖入斑馬魚胚胎中；(c)馴養魚隻，使之成長、交配及產卵；以及(d)收集魚卵、魚胚或成魚，得到富含抗病蛋白之生物體。本發明另係關於一種核酸建構物，其包含一外源抗病蛋白之核酸序列、一與其連接之螢光蛋白核酸序列及一α肌動蛋白啟動子。本發明又係關於一種表現載體，其包含有一前述之核酸建構物。
A method for enhancing production of pathogen-resistant proteins using bioreactor comprising: (a) constructing a plasmid which comprises a nucleic acid sequence of exogenous pathogen-resistant protein, a nucleic acid sequence of fluorescence protein, a nucleic acid sequence of protease-cutting site and an α-actin promoter; (b) transferring the constructed plasmid into fish embryo(s) of selected line after linearization; (c) domesticating the fish to enable to grow, mate and spawn; and (d) collecting fish egg(s), fish embryo(s) and adult fish(s) to obtain pathogen-resistant protein-rich organism(s) is described. A nucleic acid construct comprising an α-actin promoter, a nucleic acid sequence of exogenous pathogen-resistant protein and a nucleic acid sequence of fluorescence protein which are operably linked in the 5′ to 3′ direction is also presented. An expression vector comprising said nucleic acid construct is further described.
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| 專利簡述 / Intellectual Properties： |
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聯繫方式 / Contact： |
| 臺大產學合作總中心 / Center of Industry-Academia Collaboration, NTU |
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| Email：ordiac@ntu.edu.tw |
電話/Tel：02-3366-9945 |
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專利行銷平台